Journal: Pediatric Research

Article Title: Ontogeny of MMPs and TIMPs in the Murine Neocortex

doi: 10.1203/pdr.0b013e3181973aee

Figure Lengend Snippet: Figure 2. MMP-2 protein level assayed by ELISA in the neocortex of five mouse strains at embryonic (E) and postnatal (P) ages. (A, BALB/c; B, C3Hen; C, C57BL/6; D, FVB; E, Swiss; and F, summary of all strains (ANOVA 2). Square, Balb; up-triangle: C3Hen; down-triangle: FVB; rhombus: C57BL/6; and circle: Swiss). Six to ten animals were studied in duplicate at each age (males, 3–5; and females, 3–5). Bars represent mean MMP-2 protein concentration SEM. Asterisks indicate statistically significant differences from the P60 group. *p 0.05, **p 0.01 by ANOVA 1 with Dunnett’s (A–E) or Bonferroni’s (F) posttest.

Article Snippet: Quantikine human-mouse MMP-2, human TIMP-2, and mouse TIMP-1 commercial ELISA kits (R&D System, Lille, France) were used as directed.

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

Journal: Pediatric Research

Article Title: Ontogeny of MMPs and TIMPs in the Murine Neocortex

doi: 10.1203/pdr.0b013e3181973aee

Figure Lengend Snippet: Figure 4. TIMP-1 protein level determined by ELISA in the neocortex of Swiss mice at various embryonic (E) and postnatal (P) ages. Six to ten animals (males, 3–5; and females, 3–5) were studied in duplicate at each age. Bars represent mean TIMP-1 protein concentration SEM. Asterisks indi- cate statistically significant age differences from the P60 group. **p 0.01 by ANOVA 1with Dunnett’s posttest.

Article Snippet: Quantikine human-mouse MMP-2, human TIMP-2, and mouse TIMP-1 commercial ELISA kits (R&D System, Lille, France) were used as directed.

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

Journal: Nature Communications

Article Title: A nanosystem targeting tissue inhibitor of metalloproteinase-1 for continuous spatiotemporal idiopathic pulmonary fibrosis therapy

doi: 10.1038/s41467-026-68398-0

Figure Lengend Snippet: a Schematic illustration of the experimental process. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/24749x6 ). b IHC staining and quantification of TIMP-1 and COL1A1 in lung sections from non-IPF controls (n = 10 non-IPF patients) and IPF patients (n = 8 IPF patients). c Correlation analysis of TIMP-1 and COL1A1. d Schematic illustration of the experimental process. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/chpxtxr ). e IHC staining and quantification of TIMP-1 and COL1A1 in lung sections from controls and bleomycin-induced fibrosis mouse models. f Correlation analysis of TIMP-1 and COL1A1 (n = 20 samples). Immunofluorescence (IF) staining of ROS, IHC staining of AQP5, and the quantification of positive area in lung sections from non-IPF controls (n = 5 non-IPF patients for ROS; n = 10 non-IPF patients for AQP5) and IPF patients (n = 10 IPF patients for ROS; n = 8 IPF patients for AQP5) ( g ), as well as bleomycin-induced fibrosis mouse models (n = 10 mice) ( h ). Data indicate mean ± SEM, two-tailed unpaired Student’s t test. Source data are provided as a file.

Article Snippet: Rabbit anti-human CD63/CD9/ALIX antibody, HRP-conjugated goat anti-rabbit IgG(H+L) secondary antibody, Tetramethylrhodamine (TRITC)/FITC-conjugated goat anti-rabbit secondary antibody, rabbit anti-human TIMP-1 antibody, and mouse TIMP-1 ELISA kit were purchased from Proteintech (Wuhan, China).

Techniques: Immunohistochemistry, Immunofluorescence, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: A nanosystem targeting tissue inhibitor of metalloproteinase-1 for continuous spatiotemporal idiopathic pulmonary fibrosis therapy

doi: 10.1038/s41467-026-68398-0

Figure Lengend Snippet: a Illustration of the mechanism of aT for MMP/TIMP-1 rebalance. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/5c51mwj ). b , c The levels of TIMP-1 and active MMP-9 in MLF supernatants. d , e Cell apoptosis and quantification of A549 cells. f Cell viability of bleomycin-challenged A549 cells after various treatments for 24, 48, and 72 h. g Images of wound healing assay and quantification of migration area. h CLSM images, 3D maps and quantification of ROS in A549 cells. Data indicate mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test; Measurements in Fig. were taken from 3 biological replicates. Source data are provided as a Source Data file.

Article Snippet: Rabbit anti-human CD63/CD9/ALIX antibody, HRP-conjugated goat anti-rabbit IgG(H+L) secondary antibody, Tetramethylrhodamine (TRITC)/FITC-conjugated goat anti-rabbit secondary antibody, rabbit anti-human TIMP-1 antibody, and mouse TIMP-1 ELISA kit were purchased from Proteintech (Wuhan, China).

Techniques: Wound Healing Assay, Migration

Journal: Nature Communications

Article Title: A nanosystem targeting tissue inhibitor of metalloproteinase-1 for continuous spatiotemporal idiopathic pulmonary fibrosis therapy

doi: 10.1038/s41467-026-68398-0

Figure Lengend Snippet: a Illustration of animal experiment procedure. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/15qup8o ). Representative images ( b ), Masson staining ( c ), and Ashcroft score ( d ) of mouse lungs after different treatments (n = 6 per group). e IF staining of α-SMA and COL1A1 in mouse lung sections (n = 6 per group). f HYP content in mouse lung tissues (n = 6 per group). The level of TIMP-1 ( g ) and active MMP-9 ( h ) in mouse BALF (n = 4 per group). i Flow cytometry analysis of the ROS levels in mouse lung tissues (n = 5 per group). j TUNEL staining of mouse lung sections (n = 6 per group). Data indicate mean ± SEM; one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a file.

Article Snippet: Rabbit anti-human CD63/CD9/ALIX antibody, HRP-conjugated goat anti-rabbit IgG(H+L) secondary antibody, Tetramethylrhodamine (TRITC)/FITC-conjugated goat anti-rabbit secondary antibody, rabbit anti-human TIMP-1 antibody, and mouse TIMP-1 ELISA kit were purchased from Proteintech (Wuhan, China).

Techniques: Staining, Flow Cytometry, TUNEL Assay

Journal: Diabetologia

Article Title: Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.

doi: 10.1007/s00125-011-2093-9

Figure Lengend Snippet: Fig. 6 a Western blot for MMP2, MMP9 and γ-tubulin of protein extracts from subcutaneous injected adipose tissue, with quantification (b); murine TIMP, grey bars; PBS, black bars. c, d As above (a, b) for subcutaneous adipose tissue and (e, f) visceral adipose tissue. g Global MMP activity in adipose tissue extracts from mice injected with PBS (black bars) or TIMP1 (grey bars) after 3 weeks of high-fat diet. MMP activity was measured using a fluorescence resonance energy transfer substrate peptide; n=4. Results are representative of three independent injection experiments with independent protein preparations; n=5. Values (b, d, f, g) are mean ± SD; *p<0.05, **p<0.01, ***p<0.001, †p=0.07

Article Snippet: TIMP1 levels in human obese participants TIMP1 serum levels were measured in 20 obese men (BMI 32.9–54.9 kg/m2) using a kit (human TIMP1 Quantikine ELISA; R&D Systems).

Techniques: Western Blot, Injection, Activity Assay, Fluorescence, Förster Resonance Energy Transfer